Journal: Chinese Herbal Medicines

Article Title: Sini decoction alleviates inflammation injury after myocardial infarction through regulating arachidonic acid metabolism

doi: 10.1016/j.chmed.2023.12.004

Figure Lengend Snippet: Fig. 6. SND alleviated myocardial inflammation caused by MI through modulating AA metabolism pathway (mean ± SD, n = 5). (A) Expressions of sPLA2, COX-1 and COX-2 in cardiac tissue were measured by Western blot. (B) Expressions of 15-LOX and 5-LOX were measured by Western blot. ##P < 0.01 and ###P < 0.001 vs sham group. *P < 0.05 and **P < 0.01 vs model group.

Article Snippet: The primary antibodies including rabbit monoclonal antibody to 5 Lipoxygenase (ab169755, Abcam, Boston, MA, USA), rabbit monoclonal antibody to 15 Lipoxygenase (ab244205, Abcam, Boston, MA, USA), rabbit monoclonal antibody to NF-kB p65 (ab32536, Abcam, Boston, MA, USA), rabbit monoclonal antibody to COX-1 (ab109025, Abcam, Boston, MA, USA), rabbit monoclonal antibody to PLA2G2A (M02259-1, BOSTER, Wuhan, China), rabbit monoclonal antibody to COX-2 (66351-1-1 g, Proteintech, Wuhan, China), rabbit polyclonal antibody to Bax (50599-2-1 g, Proteintech, Wuhan, China), rabbit polyclonal antibody to Bcl-2 (26593- 1-AP, Proteintech, Wuhan, China), rabbit polyclonal antibody to Cleaved Caspase-3 (19677-1-AP, Proteintech, Wuhan, China), rabbit polyclonal antibody to TNF alpha (17590-1-AP, Proteintech, Wuhan, China), rabbit polyclonal antibody to IL-6 (DF6087, Affinity Biosciences, Cincinnati, Ohio, USA), rabbit polyclonal antibody to Beta-actin (GB11001, Servicebio, Wuhan, China).

Techniques: Western Blot

Journal: bioRxiv

Article Title: Optimal AAV capsid/promoter combinations to target specific cell types in the common marmoset cerebral cortex

doi: 10.1101/2024.05.09.593444

Figure Lengend Snippet: ( A ) Scheme to identify EGFP-expressing cell types by fIHC. Neurons and astrocytes were immunolabeled for NeuN and GFAP, respectively. Cells immunostained for Olig2 or Iba1 were identified as oligodendrocytes and microglia. Cells were detected with Hoechest 33342, NucBlue, in the mounting reagent ProLong Glass. ( B ) Representative immunohistochemical images of marmoset cerebral cortex that received injection of AAV2. Two serial slices were presented: one immunolabeled for GFP, NeuN, and GFAP, and another one for GFP, Olig2, and Iba1, as indicated at each panel. Scale bar, 100 μm.

Article Snippet: Tissue sections were reacted overnight at room temperature by immersion in following primary antibodies in blocking solution (2% Donkey Serum (S30-100ML, Merck), BSA (01862-87, Nacalai Tesque, Kyoto, Japan), 0.5% Triton X-100, 0.03% NaN3 in 1 x PB): rat monoclonal anti-GFP antibody (1:1,000; 04404-84; Nacalai Tesque, Kyoto, Japan), mouse monoclonal anti-NeuN antibody (1:1,000; MAB377; Merck), rabbit polyclonal anti-GFAP antibody (1:200; GFAP-Rb-Af800; Nittobo Medical, Tokyo, Japan), rabbit polyclonal anti-S-100β antibody (1:200; S100b-Rb-Af1000, Nittobo Medical), mouse monoclonal anti-Olig2 antibody (1:500; MABN50; Merck) and rabbit polyclonal anti-Iba1 antibody (1:500; 019-19741; Fujifilm Wako Chemicals, Tokyo, Japan).

Techniques: Expressing, Immunolabeling, Immunohistochemical staining, Injection

Journal: bioRxiv

Article Title: Optimal AAV capsid/promoter combinations to target specific cell types in the common marmoset cerebral cortex

doi: 10.1101/2024.05.09.593444

Figure Lengend Snippet: ( A ) Schema depicting the AAV genome structure. ( B - C ) Immunolabeled fluorescent images of EGFP in the cerebral cortex that received injection of AAV5 ( B ) or AAVrh10 ( C ) vectors expressing EGFP by the mMBP promoter. The middle immunofluorescence images present an overlay of immunolabeling for EGFP and the oligodendrocyte marker Olig2. The bottom images are magnifications of the boxed areas in the center images. Scale bar, 100 μm. ( D - E ) Summary graphs showing the specificity ( D ) and efficiency ( E ) of oligodendrocyte transduction. The dotted line in the graph indicates a ratio of oligodendrocytes to total cells present in the marmoset cortex (Figure S3). Error bars indicate S.E.M., and dots in the graph indicate the respective values for each of the individual marmosets. Asterisks indicate statistically significant differences between the AAV5 and AAVrh10. ** p < 0.01, *** p < 0.001 by student’s t-test.

Article Snippet: Tissue sections were reacted overnight at room temperature by immersion in following primary antibodies in blocking solution (2% Donkey Serum (S30-100ML, Merck), BSA (01862-87, Nacalai Tesque, Kyoto, Japan), 0.5% Triton X-100, 0.03% NaN3 in 1 x PB): rat monoclonal anti-GFP antibody (1:1,000; 04404-84; Nacalai Tesque, Kyoto, Japan), mouse monoclonal anti-NeuN antibody (1:1,000; MAB377; Merck), rabbit polyclonal anti-GFAP antibody (1:200; GFAP-Rb-Af800; Nittobo Medical, Tokyo, Japan), rabbit polyclonal anti-S-100β antibody (1:200; S100b-Rb-Af1000, Nittobo Medical), mouse monoclonal anti-Olig2 antibody (1:500; MABN50; Merck) and rabbit polyclonal anti-Iba1 antibody (1:500; 019-19741; Fujifilm Wako Chemicals, Tokyo, Japan).

Techniques: Immunolabeling, Injection, Expressing, Immunofluorescence, Marker, Transduction

Journal: Frontiers in Pharmacology

Article Title: Clemastine Ameliorates Perioperative Neurocognitive Disorder in Aged Mice Caused by Anesthesia and Surgery

doi: 10.3389/fphar.2021.738590

Figure Lengend Snippet: Effects of clemastine on expression levels of OLIG2 and MBP in hippocampus of aged mice after anesthesia and surgery. ( A ) Relative mRNA expressions of OLIG2 and MBP, normalized to that of the GAPDH internal control. ( B ) Representative western blot images of OLIG2 and MBP. ( C ) Relative protein expressions of OLIG2 and MBP, normalized to that of the β-tubulin internal control. The data are presented as mean ± S.D. ( n = 10 mice per group). * p < 0.05 compared with the CON group. **** p < 0.001 compared with the CON group. ### p < 0.005 compared with the PND group. #### p < 0.001 compared with the PND group.

Article Snippet: Rabbit polyclonal anti-mouse TNF-α antibody (1:1,000, AF8208, Beyotime, CHN), rabbit polyclonal anti-mouse IL-1β antibody (1:1,000, AF7209, Beyotime, CHN), rabbit polyclonal anti-mouse WNT10B antibody (1:1,000, DF9038, Affinity, CHN), rabbit polyclonal anti-mouse β-catenin antibody (1:1,000, AF5126, Beyotime, CHN), rabbit monoclonal anti-mouse OLIG2 antibody (1:1,000, AF1312, Beyotime, CHN), rabbit polyclonal anti-mouse MBP antibody (1:1,000, BA0094, Boster, CHN), rabbit polyclonal anti-mouse SNAP25 antibody (1:1,000, AF8016, Beyotime, CHN), rabbit monoclonal anti-mouse PSD95 antibody (1:1,000, AF1096, Beyotime, CHN), rabbit monoclonal anti-mouse BDNF antibody (1:1,000, AF1423, Beyotime, CHN) and rabbit monoclonal anti-mouse β-tubulin antibody (1:1,500, AF1216, Beyotime, CHN) were the primary antibodies used.

Techniques: Expressing, Control, Western Blot